Organelle targeting vectors
- Tools for labeling of subcellular structure
- Multi-labeling cells with different colors
CoralHue® Organelle targeting vectors allowing to visualize subcellular structures. These vectors encode fusions of fluorescent protein variants and localization signals.It can be used for fluorescent labeling of subcellular structure in living cells.
Table: CoralHue® Organelle Targeting Vectors
| Targeted subcellular structure |
Color variations |
Fluorescence proteins |
Localization tag (Targeting sequence) |
Localization | Potential applications |
|---|---|---|---|---|---|
| Plasma Membrane(PM) |
Green Orange Red |
Azami-Green(mAG1) Kusabira-Orange(mKO1) Keima-Red(mKeima-Red) |
Lyn A Protein tyrosine kinase [Rattus norvegicus] |
Inner plasma membrane | ・Track membrane dynamics. ・Monitor membrane-associated |
| Mitochondria(MT) | Green Orange Red |
Azami-Green(mAG1) Kusabira-Orange(mKO1) Keima-Red(mKeima-Red) |
Cytochrome c oxidase polypeptide IV [Saccharomyces |
Mitochondrial inner membrane |
・Track mitochondria dynamics. |
| Endoplasmic reticulum(ER) | Green Orange |
Azami-Green(mAG1) Kusabira-Orange(mKO1) |
Calreticulin;KDEL retrieval sequence[Bos taurus] |
Lumen of the endoplasmic reticulum | ・Track morphology and intracellular distribution. |
| Nucleoplasm(NP) | Green Orange Red |
Azami-Green(AG) Kusabira-Orange(KO1) Keima-Red(hdKeima-Red) |
Poly(ADP-ribose) Polymerase (PARP)[Homosapiens] |
Intranuclear | ・Track cell lineage. ・Monitor cell growth and division. |
Plasma membrane (PM)

Mitochondria (MT)

Endoplasmic reticulum (ER)

Nucleoplasm (NP)

Dual-labeling cells with CoralHue® fluorescent proteins

Multi-labeling cell with CoralHue® fluorescent proteins
