Kusabira-Orange: KO
- Bright orange fluorescence
- High pH stability
- Monomer / dimer
CoralHue® Kusabira-Orange (KO1), from the stony coral (kusabira-ishi in Japanese), absorbs light maximally at 548
nm and emits orange light at 561 nm. KO1 rapidly matures to form a fluorescent dimeric
complex. KO1 can be used to mark cells or to report gene expression without problems stemming from
protein aggregation.
CoralHue® monomeric Kusabira Orange (mKO1) maintains the brilliance and pH stability of the parent protein.
mKO1 can be used to label proteins or subcellular structures or for FRET analysis.
CoralHue® mKO2 is a mutant of mKO1 that features rapid maturation. mKO2 can be used to label
proteins or subcellular structures or for reporter assays.
Fluorescent properties
Download KO1 spectra Ex, Em (txt.)
Download mKO1 spectra Ex, Em (txt.)
Download mKO2 spectra Ex, Em (txt.)
| CHARACTERISTIC | KO1 | mKO1 | mKO2 |
|---|---|---|---|
| Oligomerization | Dimer | Monomer | Monomer |
| Number of amino acid | 218 | 218 | 218 |
| Excit./Emiss. maxima (nm) | 548/561 | 548/559 | 551/565 |
| Molar Extinction Coefficient (M-1cm-1) | 73,700 (548 nm) | 51,600 (548 nm) | 63,800 (551 nm) |
| Fluorescence quantum yield | 0.45 | 0.60 | 0.62 |
| Brightness*1 | 33.2 | 31.0 | 39.6 |
| pH sensitivity | pKa<5.0 | pKa<5.0 | pKa=5.5 |
| Cytotoxicity*2 | Not observed | Not observed | Not observed |
| Resistance to PFA fixation | Not tested | Not tested | +++ |
*1Brightness: Molar Extinction Coefficient ×Fluorescence Quantum Yield / 1000
*2Toxicity when expressed in HeLa cells
Performance and use
Kusabira-Orange are useful for labeling organelle.
Kusabira-Orange is easily expressed and detected in a wide range of
organisms. It has been demonstrated in
nucleoplasm, plasma membrane, endoplasmic reticulum and mitochondrial targeting signal models.
Kusabira-Orange is a useful protein for expression into various species.
 |
 |
 |
 |
KO1 TG pig Provided by Dr. Nagashima |
mKO1 fused with Vasa protein expressed in Drosophila ovary (red). Provided by Dr. Nakamura RIKEN |
The olfactory bulb of zebrafish Provided by Dr. Miyasaka and Dr. |
KO1 expressed in the epidermis of onion Provided by Dr. Iida and Dr. Hoshino |
Recommended antibodies
CoralHue® KO1, mKO1 and mKO2 can be recognized using antibodies as shown below.
- Anti-monomeric Kusabira-Orange 1 for WB, Code No. M104-3
- Anti-monomeric Kusabira-Orange 1 for IP, Code No. M105-3
- Anti-monomeric Kusabira-Orange 2 for WB, IP, IC and IH, Code No. M168-3
- Anti-monomeric Kusabira-Orange 2 for WB, IP, IC and IH, Code No. PM051
WB: Western blotting, IP: Immunoprecipitation, IC: Immunocytochemistry, IH: Immunohistochemistry
References
Cyan-emitting and orange-emitting fluorescent proteins as a donor/acceptor pair for fluorescence resonance energy transfer.
Karasawa S, Araki T, Nagai T, Mizuno H, Miyawaki A.
Biochem J. (2004) 381(Pt 1):307-12.
PMID: 15065984
Transgenic-Cloned Pigs Systemically Expressing Red Fluorescent Protein, Kusabira-Orange
Hitomi Matsunari, Masafumi Onodera, Norihiro Tada, Hideki Mochizuki, Satoshi Karasawa, Erika Haruyama, Naoki Nakayama, Hitoshi Saito, Satoshi Ueno, Mayuko Kurome, Atsushi Miyawaki, Hiroshi Nagashima
Cloning and Stem Cells. (2008) 10: 313-324.
PMID: 18729767
