Azami-Green: AG
- Bright green fluorescence
- High pH stability
- Monomer/tetramer
CoralHue® Azami-Green (AG), cloned from the stony coral (azami-sango in Japanese), absorbs light maximally at 492 nm and emits green light at 505 nm. AG is stable in the biological pH range and does not show a significant loss of fluorescent signal, making it advantageous over other fluorescent proteins such as EGFP. And AG also matures rapidly to form tetramers that are brighter than EGFP
CoralHue® monomeric Azami-Green (mAG1) maintains the brightness and pH stability of the parent protein AG. mAG1 can be used for labeling proteins or subcellular structures.
Fluorescent properties
Download AG spectra Ex, Em (txt.)
Download mAG1 spectra Ex, Em (txt.)
| CHARACTERISTIC | AG | mAG1 |
|---|---|---|
| Oligomerization | Tetramer | Monomer |
| Number of amino acid | 225 | 225 |
| Excit./Emiss. Maxima (nm) | 492/505 | 492/505 |
| Molar extinction coefficient (M-1cm-1) | 72,300 (492 nm) | 55,500 (492 nm) |
| Fluorescence quantum yield | 0.67 | 0.74 |
| Brightness *1 | 48.4 | 41.0 |
| pH sensitivity | pKa<5.0 | pKa=5.8 |
| Cytotoxicity *2 | No | No |
*1Brightness: Molar Extinction Coefficient ×Fluorescence Quantum Yield / 1000
*2Toxicity when expressed in HeLa cells
Performance and use
AG is resistant to acidity
AG shows high resistance in a wide range of biological pH. This allows AG to be used identify cells or to report gene expression without being influenced by pH change. The pH dependence of both CoralHue® AG and mAG1 was identical to that of its absorption spectra.
AG and mAG1 are useful for labeling organelle.
AG and mAG1 performance in fusions has been demonstrated in the nucleoplasm, plasma membrane, ER and mitochondria-targeting signal models. We offer these constructs as targeting vector series.
| Nucleoplasm targeting-AG | |
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mAG1 is available for protein labeling
mAG1 performance in fusion has been demonstrated in p80 coilin, SC35 and fibrillarin.
Recommended antibodies
CoralHue® AG and mAG1 can be recognized using antibodies as shown below.
- Anti-monomeric Azami-Green 1 for WB, Code No. M102-3
- Anti-Azami-Green for WB, Code No. PM011
- Anti-Azami-Green for IP, Code No. M103-3
- Anti-monomeric Azami-Green 1 for WB, IP, IC and IH. Code No. PM052
WB: Western blotting, IP: Immunoprecipitation, IC: Immunocytochemistry, IH: Immunohictochemistry
References
1: A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling.
Karasawa S, Araki T, Yamamoto-Hino M, Miyawaki A.
J Biol Chem. (2003) 278:34167-71.
PMID: 12819206
2: Crystallization and preliminary X-ray analysis of a monomeric mutant of Azami-Green (mAG), an Aequorea victoria green fluorescent protein-like green-emitting fluorescent protein from the stony coral Galaxea fascicularis.
Ebisawa T, Yamamura A, Kameda Y, Hayakawa K, Nagata K, Tanokura M.
Acta Crystallogr Sect F Struct Biol Cryst Commun. (2009) 65(Pt 12):1292-5.
PMID: 20054132
