Anti- CoralHue® fluorescent protein antibodies


We offer a wide variety of anti-CoralHue fluorescent protein antibodies.

Fluorescent protein Product name WB IP IC IH clone Isotype Code No.
Midoriishi-Cyan anti-midoriishi-Cyan ◯       5B7 Mouse lgG1 M130-3
anti-midoriishi-Cyan   ◯     2C1 Mouse lgG2b M116-3
Azami-Green anti-monomeric Azami-Green 1 ◯       2F11 Mouse lgG1k M102-3
anti-Azami-Green ◯       polyclonal Rabbit lgG PM011
anti-Azami-Green   ◯     3D10 Mouse lgG2ak M103-3
anti-monomeric Azami-Green 1 ◯ ◯ ◯ ◯ polyclonal Rabbit lg(aff.) PM052
Kusabira-Orange anti-monomeric Kusabira-Orange 1 ◯       1H7 Mouse lgG1k M104-3
anti-monomeric Kusabira-Orange 1   ◯     2G9 Mouse lgG1k M105-3
anti-monomeric Kusabira-Orange 2 ◯ ◯ ◯ ◯ 3B3 Mouse lgG1k M168-3
anti-monomeric Kusabira-Orange 2 ◯ ◯ ◯ ◯ polyclonal Rabbit lg(aff.) PM051
Kusabira-Green anti-monomeric Kusabira-Green
N-terminal fragment		 ◯       1E6 Mouse lgG2b M148-3
anti-monomeric Kusabira-Green
C-terminal fragment		 ◯       21B10 Mouse lgG2a M149-3
Keima-Red anti-monomeric Keima-Red ◯       2F7 Mouse lgG2a M126-3
anti-Keima-Red   ◯     3C9 Mouse lgG1 M127-3
Dronpa-Green anti-Dronpa-Green ◯       4D12 Mouse lgG2a M117-3
anti-Dronpa-Green   ◯     2F6 Mouse lgG2b M118-3
Kaede anti-Kaede ◯       3B1 Mouse lgG1 M125-3
anti-Kaede ◯       polyclonal Rabbit lgG PM012
anti-Kaede   ◯     2F4 Mouse lgG1k M106-3
Kikume Green-Red anti-Kikume Green-Red ◯       5B3 Mouse lgG2b M128-3
anti-Kikume Green-Red   ◯     2D3 Mouse lgG2b M129-3

Click on the product number to get the instruction manual for the product (PDF format).
WB: western blotting, IP: immunoprecipitation, IC: immunocytochemistry, IH: immunohistochemistry


Antibodies cross-reactivity information

Fluorescent protein Product name WB Western Blot
Cross reactivity		 clone Isotype Code No.
Midoriishi-Cyan anti-midoriishi-Cyan ◯ MiCy, mMiCy 5B7 Mouse lgG1 M130-3
anti-midoriishi-Cyan     2C1 Mouse lgG2b M116-3
Azami-Green anti-monomeric Azami-Green 1 ◯ mAG1 2F11 Mouse lgG1k M102-3
anti-Azami-Green ◯ AG, mAG1 polyclonal Rabbit lgG PM011
anti-Azami-Green     3D10 Mouse lgG2ak M103-3
anti-monomeric Azami-Green 1 ◯ mAG1 polyclonal Rabbit lg(aff.) PM052
Kusabira-Orange anti-monomeric Kusabira-Orange 1 ◯ mKO1, mKO2, mKG, mKG-O(1), mKOkappa(2) 1H7 Mouse lgG1k M104-3
anti-monomeric Kusabira-Orange 1     2G9 Mouse lgG1k M105-3
anti-monomeric Kusabira-Orange 2 ◯ mKO2, mKG, mKG, mKG-O(1), mKOkappa(2) 3B3 Mouse lgG1k M168-3
anti-monomeric Kusabira-Orange 2 ◯ KO1, mKO1, mKO2, mKG, mKG, mKG-O(1), mKOkappa(2) polyclonal Rabbit lg(aff.) PM051
Kusabira-Green anti-monomeric Kusabira-Green
N-terminal fragment		 ◯ mKO1, mKO2, mKG 1E6 Mouse lgG2b M148-3
anti-monomeric Kusabira-Green
C-terminal fragment		 ◯ mKO2, mKG 21B10 Mouse lgG2a M149-3
Keima-Red anti-monomeric Keima-Red ◯ mKeima-Red 2F7 Mouse lgG2a M126-3
anti-Keima-Red     3C9 Mouse lgG1 M127-3
Dronpa-Green anti-Dronpa-Green ◯ DG1, DG3 4D12 Mouse lgG2a M117-3
anti-Dronpa-Green     2F6 Mouse lgG2b M118-3
Kaede anti-Kaede ◯ Kaede 3B1 Mouse lgG1 M125-3
anti-Kaede ◯ Kaede polyclonal Rabbit lgG PM012
anti-Kaede     2F4 Mouse lgG1k M106-3
Kikume Green-Red anti-Kikume Green-Red ◯ KikGR, mKikGR 5B3 Mouse lgG2b M128-3
anti-Kikume Green-Red     2D3 Mouse lgG2b M129-3

(1) Age-dependent preferential dense-core vesicle exocytosis in neuroendocrine cells revealed by newly developed monomeric fluorescent timer protein.
Tsuboi T, Kitaguchi T, Karasawa S, Fukuda M, Miyawaki A.
Mol Biol Cell. (2010) 21:87-94.
PMID: 19889833

(2) Improving membrane voltage measurements using FRET with new fluorescent proteins.
Tsutsui H, Karasawa S, Okamura Y, Miyawaki A.
Nat Methods. (2008) 5:683-5.
PMID: 18622396.



Antibodies for detection of FUCCI TG mice!!


Anti-monomeric Kusabira-Orange 2 (polyclonal antibody): code No. PM051

PM051 is available for immunostaining of “Fucci-G1 Orange” (Fucci; Fluorescent Ubiquitination-based Cell Cycle Indicator). Fucci-G1 Orange encodes CoralHue® monomeric Kusabira-Orange2 (mKO2) fused to a part of human Cdt1 (hCdt1: Cdc10 dependent transcript 1). It is possible to use PM051 for Fucci transgenic strain, B6.Cg-Tg(Fucci)596Bsi mice which express Fucci-G1 Orange.

Immunohistochemical detection of mKO2 on frozen section of B6.Cg-Tg (Fucci) 596Bsi mouse embryonic brain (E12) with PM051 (1) and Fucci-G1 Orange own fluorescence (2). Fluorescence microscope: Axiovert200
Filter set:
1, Carl Zeiss Filter sets No.26 (for Alexa Fluor® 647)
2, OLYMPUS FSET-KOHQ (for mKO2)

Immunocytochemical detection of mKO2 in Fucci-G1 Orange transfected HeLa with PM051.
Fluorescence microscope: Axiovert200

Filter set:
3, Carl Zeiss Filter sets No.26 (for Alexa Fluor® 647)
4, OLYMPUS FSET-KOHQ (for mKO2)

3:Anti-mKO2
4: FucciG1Orange
5: Transmission light



Anti-monomeric Azami-Green 1 (polyclonal antibody): code No. PM052

PM052 is available for immunostaining of “Fucci-S/G2/M Green” (Fucci; Fluorescent Ubiquitination-based Cell Cycle Indicator). Fucci-S/G2/M Green encodes CoralHue® humanized monomeric Azami-Green1 (hmAG1) fused to a part of human Geminin (hGeminin). It is possible to use PM052 for Fucci transgenic strain, B6.Cg-Tg(Fucci)504Bsi mice which express Fucci-S/G2/M Green.

Immunohistochemical detection of mAG1 on frozen section of B6.Cg-Tg (Fucci) 504Bsi mouse embryonic brain (E13) with PM052 (1) and Fucci-S/G2/M Green own fluorescence (2).
Fluorescence microscope: Axiovert200
Filter set:
1, Carl Zeiss Filter sets No.26 (for Alexa Fluor® 647)
2, OLYMPUS U-MGFP-HQ (for mAG1)

Immunocytochemical detection of mAG1 in Fucci-S/G2/M Green transfected HeLa with PM052.
Fluorescence microscope: Axiovert200
Filter set:
3, Carl Zeiss Filter sets No.26 (for Alexa Fluor® 647)
4, OLYMPUS U-MGFP-HQ (for mAG1)

3:Anti-mAG1
4: Fucci-S/G2/M Green
5: Transmission light



Anti-monomeric Kusabira-Orange 2 (monoclonal antibody): code No. M168-3

M168-3 is available for immunostaining of “Fucci-G1 Orange ” (Fucci; Fluorescent Ubiquitination-based Cell Cycle Indicator). Fucci-G1 Orange encodes CoralHue® monomeric Kusabira-Orange2 (mKO2) fused to a part of human Cdt1 (hCdt1: Cdc10 dependent transcript 1). It is possible to use M168-3 for Fucci transgenic strain, B6.Cg-Tg(Fucci)596Bsi mice which express Fucci-G1 Orange .

Immunohistochemical detection of mKO2 on frozen section of B6.Cg-Tg (Fucci) 596Bsi mouse embryonic brain (E12) with M168-3 (1) and Fucci-G1 Orange own fluorescence (2).
Fluorescence microscope: Axiovert200
Filter set:
1, Carl Zeiss Filter sets No.26 (for Alexa Fluor® 647)
2, OLYMPUS FSET-KOHQ (for mKO2)

Immunocytochemical detection of mKO2 in Fucci-G1 Orange transfected HeLa with M168-3.
Fluorescence microscope: Axiovert200
Filter set:
3, Carl Zeiss Filter sets No.26 (for Alexa Fluor® 647)
4, OLYMPUS FSET-KOHQ (for mKO2)

3:Anti-mKO2
4: Fucci-G1 Orange
5: Transmission light